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There are 3 messages in this issue.
Topics in this digest:
1. BELL PEPPER LEAF CURL VIRUS - PAKISTAN (LAHORE)
From: Jawad Asghar <jawad@alumni.washington.edu>
2. UNICEF India Internship (Children's Health)] (fwd)
From: Jawad Asghar <jawad@alumni.washington.edu>
3. HIV Mortality Decreases in India after introduction of generic HAART in India
From: Jawad Asghar <jawad@alumni.washington.edu>
________________________________________________________________________
________________________________________________________________________
Message: 1
Date: Sat, 22 Oct 2005 17:33:00 -0400
From: Jawad Asghar <jawad@alumni.washington.edu>
Subject: BELL PEPPER LEAF CURL VIRUS - PAKISTAN (LAHORE)
BELL PEPPER LEAF CURL VIRUS - PAKISTAN (LAHORE)
*************************************
A ProMED-mail post
<http://www.promedmail.org>
ProMED-mail is a program of the
International Society for Infectious Diseases
<http://www.isid.org>
Sponsored by Elsevier, publisher of
Tropical Infectious Diseases, 2nd Edition
<http://thelancet.url123.com/a5445>
Date: 20 Oct 2005
From: ProMED-mail<promed@promedmail.org>
Source: British Society for Plant Pathology, New Disease Reports,
Vol. 12 [edited]
<http://www.bspp.org.uk/ndr/jan2006/2005-87.asp>
1st report of a begomovirus associated with leaf curl disease of bell
pepper in Pakistan
M. Tahir <tahirsbs@yahoo.com> and M.S. Haider, School of Biological
Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore,
Pakistan Accepted for publication 18 Oct 2005.
Bell pepper (_Capsicum annuum_ var. _grossum_; family _Solanaceae_)
is a vegetable commonly cultivated in most of the vegetable growing
areas of Pakistan. Leaf samples from 3 bell pepper plants showing
leaf curl symptoms and from 2 apparently healthy (symptomless) plants
were collected during a recent survey for begomoviruses, from the
vegetable fields around the Lahore city. Overall disease incidence
was 60-70 percent with severe leaf curl symptoms.
DNA was extracted from both types of samples. The presence of a
begomovirus was confirmed by PCR amplification using a degenerate
primer pair, designed to conserved regions of the coat protein genes
from published sequences of begomoviruses from the Old World (Haider
et al., 2005; virion-sense primer 5' ATG(C/A/T)(G/C)
(G/C/A)AAGCG(A/T)(C/A)C(A/C)G(G/C)(A/C) GATAT-3'; complementary-sense
primer 5' TTAATT (T/G/C)(C/G/A)(A/T/C)(A/T/G) A(C/T)
(A/T/C)(G/C)(C/A/T)(A/G)TCATA(G/A)AA(A/G)TA-3').
An amplification product of the expected size for the coat protein
gene (approx. 750 bp) was produced from symptomatic samples but there
was no amplification from the symptomless plants. The PCR product was
cloned and sequenced. The sequences obtained from bell pepper
(Accession No. AM051090) showed the highest levels of sequence
identity to Tomato leaf curl New Delhi virus - [Pakistan:_Solanum_]
(syn. Solanum yellow leaf curl virus) segment A (84 percent over a
stretch of 656 nucleotides, Accession number AJ620187). Since the
coat protein gene is the most conserved gene among
whitefly-transmitted geminiviruses (Padidam et al., 1995), it is
predicted that overall homology for the complete genome may be even
less than 84 percent.
These findings indicate that the virus of bell pepper is a new
species of begomovirus for which we suggest the name Bell pepper leaf
curl virus (BPLCV).
Recent reports have shown that many begomoviruses of the Old World
are associated with a single-stranded DNA satellite (DNA beta;
Briddon et al., 2003). Attempts to identify the presence of a DNA
beta in the infected _Capsicum annuum_ var. _grossum_ samples, using
universal DNA beta primers (Briddon et al., 2002) produced a ca.
1.4-kb product, corresponding to that expected for a full-length
amplicon from a satellite. This has not been sequenced but probably
indicates that BPCLV is associated with a DNA satellite.
To the best of our knowledge this the 1st report of a begomovirus
associated with leaf curl disease of _Capsicum annuum_ var. _grossum_.
Acknowledgements
The authors would like to thank Dr Rob Briddon (Higher Education
Commission [Pakistan] - Foreign Faculty Hiring Programme) for
guidance in sequencing analysis.
References
Briddon RW, Bull SE, Amin I, Idris AM, Mansoor S, Bedford ID, Dhawan
P, Rishi N, Siwatch SS, Abdel-Salam AM, Brown JK, Zafar Y, Markham
PG, 2003. Diversity of DNA beta; a satellite molecule associated with
some monopartite begomoviruses. Virology 312, 106-121.
Briddon RW, Bull SE, Mansoor S, Amin I, Markham PG, 2002. Universal
primers for the PCR-mediated amplification of DNA beta; a molecule
associated with some monopartite begomoviruses. Molecular
Biotechnology 20, 315-318.
Haider MS, Tahir M, Latif S, Briddon RW, 2005. First report of Tomato
leaf curl New Delhi virus infecting Eclipta prostrata in Pakistan.
New Disease Reports [<http://www.bspp.org.uk/ndr/>] Volume 11
Padidam M, Beachy RN, Fauquet CM, 1995. Classification and
identification of geminiviruses using sequence comparisons. Journal
of General Virology 76:249-263.
- --
ProMED-mail
<promed@promedmail.org>
[This report demonstrates that begomoviruses are continually evolving
in Asia, especially in India and Pakistan. The combination of high
temperatures, presence of high populations of whiteflies, and
suitable natural host plants apparently results in a mix that is
conducive to generating new begomovirus strains in the region. The
begomovirus-beta satellite complexes referred to above are associated
with economically important diseases and have been isolated from
vegetable and fiber crops, ornamental plants, and weeds throughout
Africa and Asia. Their widespread distribution and diversity, coupled
to the global movement of plant material and the dissemination of the
whitefly vector, suggests that these disease complexes pose a serious
threat to tropical and sub-tropical agro-ecosystems worldwide.
Collaborative work which is described in the 2nd link below,
involving research scientists at the Plant Biotechnology Division,
National Institute for Biotechnology and Genetic Engineering (NIBGE),
PO Box 577, Jhang Road, Faisalabad, Pakistan and the Department of
Disease and Stress Biology, John Innes Centre, Colney, Norwich, UK
NR4 7UH, contains information suggesting that these disease complexes
are rapidly expanding in geographical distribution and host range. As
an example, cotton leaf curl disease, originally a major problem in
central Pakistan, is now causing extensive damage in India. In the
same region, new diseases are emerging in crops such as tomato,
tobacco, chillies and papaya. The presence of such a diverse
population of begomoviruses in a single region, coupled with the
propensity of these viruses to exchange genetic material by
recombination [35,37-39], increases the probability of new virus
diseases emerging in the region.
These viruses will likely cause epidemics in previously unaffected
crops, a problem that will be exacerbated by the selective use of
elite cultivars, movement of infected plant material and widespread
introduction of the whitefly vector (_Bemisia tabaci_). Continued
growth in international trade and travel could result in these
whitefly-transmissible disease complexes reaching the New World, as
was demonstrated when Tomato yellow leaf curl virus (TYLCV-Is)
appeared in the eastern Caribbean in Cuba, the Dominican Republic,
and Jamaica. In the case of the Dominican Republic, it was believed
to have been introduced on infected but asymptomatic tomato
transplants from the eastern Mediterranean region. An isolate of
TYLCV-Is from the Dominican Republic and 98 percent identical to an
isolate from Israel appeared to have entered the United States in
Dade County, Florida, in late 1996 or early 1997. Subsequently,
infected tomato transplants produced for retail sale at 2 Dade County
facilities were rapidly distributed via retail garden centers
throughout the state.
Links:
<http://www.danforthcenter.org/iltab/pdf/Fauquet_Phyto_05.pdf>
<http://www.jic.ac.uk/staff/john-stanley/satellite/TIPSreview.pdf>
<http://www.apsnet.org/pd/PDFS/1999/0920-02S.pdf>
- - Mod.DH]
--
Rana Jawad Asghar MD. MPH.
Coordinator South Asian Public Health Forum
jawad@alumni.washington.edu http://www.DrJawad.com
Typhoid Net http://www.typhoid.net
________________________________________________________________________
________________________________________________________________________
Message: 2
Date: Fri, 21 Oct 2005 18:04:47 -0400
From: Jawad Asghar <jawad@alumni.washington.edu>
Subject: UNICEF India Internship (Children's Health)] (fwd)
---------- Forwarded message ----------
From: Ann Marie Kimball <akimball@u.washington.edu>
Date: Oct 21, 2005 1:37 PM
Subject: [Ihp] [Fwd: FW: UNICEF India Internship (Children's Health)] (fwd)
To: ihp@u.washington.edu
of interest.
"Make something happen..."
Ann Marie Kimball, MD,MPH,FACPM
Director, Asia Pacific Economic Cooperation Emerging Infections
Network,http://depts.washington.edu/einet
Director, "Amauta" Global Informatics Research and Training Program
http://depts.washington.edu/infomatx/
Professor
Epidemiology, Health Services
Adjunct Professor, Biomedical and Health Informatics and Medicine
School of Public Health and Community Medicine, University of Washington
BOX 357236, Seattle, 98195.
phone:
(206)616-2949, cell (206)947-0779
FAX (206)616-9415
---------- Forwarded message ----------
Date: Fri, 21 Oct 2005 08:49:30 -0700 (Pacific Daylight Time)
From: Martina Morris <morrism@u.washington.edu>
To: morrism@u.washington.edu
Subject: [Fwd: FW: UNICEF India Internship (Children's Health)] (fwd)
> UNICEF INDIA INTERNSHIP PROGRAMME
>
> 12 June - 18 August 2006
>
> Background
> UNICEF India Country Office invites applications for a full-time India
> Internship programme from 12 June to 18 August 2006. Conceived as part of a
> broader initiative to build a Knowledge Community for Children in India
> (KCCI), UNICEF India is partnering with research and academic institutions to
> encourage young students and scholars from around the world in engaging in
> development issues pertaining to India's children and women.
>
> The internship offers an opportunity for analysis, research and
> documentation (written as well as film) of field level interventions for
> children in India across a variety of areas: child development, child
> protection, health, nutrition, primary education, and water and sanitation.
> The assignment will involve a combination of desk-work and field work at the
> district or village level. The internship will provide an opportunity for
> young graduate students from India and abroad to work in groups under the
> supervision of a partner research institution.
>
> Expected Outputs
> The expected outputs from each team are: (a) 30 page written case study
> (b) A ten minute film clip documented by a trained film student in each team.
> On meeting minimum quality standards, these will be published and
> disseminated.
>
> Eligibility
> Applicants should be enrolled in a Masters level graduate programme or a
> higher degree program, in one of the following disciplines: anthropology,
> child psychology, demography, economics, education, engineering, film making,
> human rights, international development, journalism, legal studies, public
> health, sociology, statistics, rural development, social work, or any other
> relevant area. Those who have completed their Masters level graduate
> programmes in 2006 are also eligible to apply. Applicants should have
> excellent command of English, both written and spoken. Applicants for whom
> English is not the first language will be asked to provide standard test
> scores such as TOEFL scores.
>
> Location
> Selected interns will be placed in research institutions across states
> where UNICEF has a presence. Likely locations are: Hyderabad (Andhra
> Pradesh), Patna (Bihar), Gandhinagar (Gujarat), Bhopal (Madhya Pradesh),
> Mumbai (Maharashtra), Bhubhaneswar (Orissa), Chennai (Tamil Nadu), Kolkata
> (West Bengal), Jaipur (Rajasthan), and Lucknow (Uttar Pradesh). Interns will
> report to New Delhi for an initial orientation before traveling to their
> assigned duty stations where they will be based and from where they will be
> expected to travel to village / district field sites.
>
> Support
> All interns are expected to bear the costs such as travel from home to
> duty station and back, as well as accommodation and food for the entire
> duration of the internship programme. UNICEF India will meet the expenses
> incurred on the following: (a) travel from Delhi to the assigned state
> capital after the orientation workshop, (b) field trips from the State
> capital to the field and, (c) travel from state capital to Delhi for the
> closing workshop at the end of the internship programme. UNICEF will also
> provide Rs. 15,000 as partial support to all interns for the two month
period.
>
> Insurance
> UNICEF does not accept responsibility for medical insurance. Interns must
> ensure that they carry appropriate medical and life insurance coverage for
> the entire period of the internship, without which they will not be able to
> participate in the programme.
>
>
> Application
>
> Interested and eligible students should download application from
> <http://www.unicef.org/about/employ/index_forms.html>http://www.unicef.org/
about/employ/index_forms.html
> (titled "Application for offices outside New York"). Students are requested
> to indicate in the cover letters their area of interest and preferred
> geographical area which will be taken into consideration to the best extent
> possible. Please note that the dates of this programme are non-negotiable and
> interns are expected to attend the entire duration of the programme.
>
>
> Due Date
> Completed applications should be sent to
> <mailto:indiahr@unicef.org>indiahr@unicef.org no later than December 15,
> 2005. Please write "UNICEF India Internship Application" in the subject of
> your email. UNICEF India will contact only the short listed candidates who
> will be subject to a phone interview. Final selection will take place no
> later than 31 March 2006.
>
>
> Andrew Coopersmith, Ph.D.
> Associate Director
> Career Services
> 3718 Locust Walk, Suite 20
> University of Pennsylvania
> Philadelphia, PA 19104-6209
> (215) 898-4381
> www.vpul.upenn.edu/careerservices
Jennifer S. Furlong, Ph.D.
Graduate Student/Postdoctoral Fellow Career Counselor
Career Services
Suite 20, McNeil Building
3718 Locust Walk
Philadelphia, PA 19104-6209
T. 215.898.7530
F. 215.898.2687
www.vpul.upenn.edu/careerservices
_______________________________________________
Ihp mailing list
Ihp@u.washington.edu
http://mailman1.u.washington.edu/mailman/listinfo/ihp
--
Rana Jawad Asghar MD. MPH.
Coordinator South Asian Public Health Forum
jawad@alumni.washington.edu http://www.DrJawad.com
Typhoid Net http://www.typhoid.net
________________________________________________________________________
________________________________________________________________________
Message: 3
Date: Sat, 22 Oct 2005 17:34:10 -0400
From: Jawad Asghar <jawad@alumni.washington.edu>
Subject: HIV Mortality Decreases in India after introduction of generic HAART in India
---------- Forwarded message ----------
From: Rakesh Kumar <kumarr@u.washington.edu>
Date: Oct 22, 2005 10:20 AM
Subject: [Ihp] FW: [AIDS-INDIA] HIV Mortality Decreases in India after
introduction of generic HAART in India
To: ihp@u.washington.edu
Friends,
I thought it might be interesting to some of us.
Rakesh.
-----Original Message-----
From: AIDS-INDIA@yahoogroups.com [mailto:AIDS-INDIA@yahoogroups.com] On
Behalf Of Phi Huynhdo
Sent: Friday, October 21, 2005 4:31 AM
To: AIDS-INDIA@yahoogroups.com
Subject: [AIDS-INDIA] HIV Mortality Decreases in India after introduction of
generic HAART in India
Dear Forum members
HIV Mortality Decreases in India
The introduction of lower-cost generic HIV drugs in
India has encouraged more people to seek treatment,
substantially dropping the rate of HIV deaths.
Many of the more than 5 million HIV-infected people in
India have been unable to obtain treatment due to the
high cost. But with the introduction of generic
anti-retroviral therapy into India in 2000, the price
of HIV drugs fell from $778 a month to $33 per month
in 2003.
That sparked a rise in the number of people seeking
and obtaining treatment. Researchers said in 1996,
only 13 percent of Indian HIV patients were able to
afford treatment. That number rose to 44 percent in
2003.
Despite the improvement offered by the ART drugs, they
also caused some side effects, such as rash, nausea,
diarrhea, headache and anemia. But patients apparently
saw the benefits outweighed the toxicities, said lead
author Dr. Nagalingeswaran Kumarasamy of the Y.R.
Gaitonde Center for AIDS Research and Education in
Chennai. He said with still yet lower prices expected
comes further encouragement for Indian HIV patients to
seek treatment.
The report is detailed in the Nov. 15 issue of
Clinical Infectious Diseases, now available online.
Source: United Press International
Here's the abstract of the original article
The Changing Natural History of HIV Disease: Before
and After the Introduction of Generic Antiretroviral
Therapy in Southern India
N. Kumarasamy,1 Suniti Solomon,1 Sreekanth K.
Chaguturu,2 Anitha J. Cecelia,1 Snigdha Vallabhaneni,2
Timothy P. Flanigan,2 and Kenneth H. Mayer2
1YRG Centre for AIDS Research and Education, Voluntary
Health Services, Chennai, India, and 2Division of
Infectious Diseases, Miriam Hospital, Brown
University, Providence, Rhode Island
The number of individuals seeking treatment for
infection with human immunodeficiency virus increased
as the cost of highly active antiretroviral therapy
(HAART) decreased 20-fold after the introduction of
generic HAART in India in the year 2000. The incidence
of tuberculosis and opportunistic infections decreased
to <2 cases per 100 person-years. Death rates
decreased from 25 to 5 deaths per 100 person-years
between 1997 and 2003.
Received 30 March 2005; accepted 11 July 2005;
electronically published 13 October 2005.
Reprints or correspondence: Dr. N. Kumarasamy,
YRG Centre for AIDS Research and Education, Voluntary
Health Services, Taramani, Chennai 600113, India
Greetings
HDP
E-mail: <huynhdophi@yahoo.com>
________________________________________________________________________
________________________________________________________________________
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http://www.saphf.org
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